Antioxidant Activity of Water and Ethyl Acetate Fractions of Qust Al Hindi Root (Saussurea lappa) Using DPPH Inhibition Method

Penulis

  • A. Musdalifah Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar 90231
  • Sukmawati Sukmawati Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar 90231
  • Masdiana Tahir Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar 90231

DOI:

https://doi.org/10.33096/pharmrep.v3i2.312

Kata Kunci:

Antioxidants, DPPH, Fraction, Qust Al Hindi (Saussurea lappa)

Abstrak

Free radicals are molecules that do not have an electron pair in the outer shell, making them highly reactive. Oxidation due to free radicals can be prevented and inhibited by substances, namely antioxidants. Natural antioxidants are obtained from plants or fruits, one of which is Qust al hindi (Saussurea lappa).  Qust al hindi is a plant native to India that is used as a traditional medicine. Qust al hindi contains various phytochemical compounds, one of which is flavonoid compounds that function as antioxidants. The purpose of this study was to analyze the antioxidant activity of water and ethyl acetate fractions of qust al hindi plant roots by 1,1-diphenyl-2picrylhydrazyl (DPPH) silencing method using UV-Vis spectrophotometry based on the calculation of IC50 value using GraphPad Prism Application. The results of this study indicate that the water and ethyl acetate fractions of qust al hindi plant roots have antioxidant activity against DPPH free radicals with IC50 values of 203.5 µg/mL; 193.3 µg/mL, respectively, which are included in the very weak and weak antioxidant groups.

Referensi

Amic, D., Beslo, D., Trinajstic, N., Davidovic. Structure-Radical Scavenging Activity Relationships of Flavonoids. Croatia Chem Acta 2003, 67

Fauzi, M. N. (2021). Uji Kualitatif dan Uji Aktivitas Antioksidan Ekstrak Etanolik Buah Maja (Aegle Marmelos (L.)Correa) dengan Metode DPPH. Jurnal Riset Farmasi, 1(1), 1–8.

Fawwaz M, Pratama M, Hasrawati A, Sukmawati, Widiastuti H, Rahmawati. Total carotenoids, antioxidant and anticancer effect of penaeus monodon shells extract. Biointerface Research in Applied Chemistry. 2020; 11(4): 11293-11302.

Fawwaz M, Pratama M, Musafirah, Wahab I, Iriani R, Aminah, Kusuma AT, Arsul MI. Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content. Indonesian Journal of Pharmaceutical Science and Technology. 2023; 10(2): 104-110.

Fitriani, N., Herman, H., & Rijai, L. (2019). Antioksidan Ekstrak Daun Sumpit (Brucea javanica (L). Merr) dengan Metode DPPH. Jurnal Sains Dan Kesehatan, 2(1), 57–62.

Kamalpreet, Loveneet Kaur, Ajaypal Singh, Jaspreet Kaur and Navjeet Kaur. 2019. A brief review of remedial uses of Saussurea lappa. Journal of Pharmacognosy and Phytochemistry 2019; 8(3): 4423-4430.

Molyneux, P. 2004. The Use Of The Stable Free Radical Diphenylpicrylhydrazyl (Dpph), For Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 26 (2): 211-219.

Qazi, M. A., & Molvi, K. I. (2018). Free Radicals and their Management. American Journal of Pharmacy And Health Research, 6(4), 1–10

Rahmawati, A. M., & Sarif, L. M. (2012). Analisi Aktivitas Antioksidan Produk Sirup Buah Mengkudu (Morinda citrifolia L.) Dengan Metode DPPH. Jurnal Fitofarmaka Indonesia, 2(2), 97–101.

Sayuti, K. Rina Yenrina., 2015. Antioksidan, Alami dan Sintetik. Andalas University Press : Padang. ISBN : 978-602-8821-97-1.

Simanjuntak. (2012). Peran Antioksidan Flavonoid Dalam Meningkatkan Kesehatan. Bina Widya, 23 nomor 3(4), 328–331.

Singh, Ravinder. KK Chahal and Nancy Singla. 2017. Chemical composition and pharmacological active ties of Saussurea lappa: A review. Journal of Pharmacognosy and Phytochemistry 2.

Sukmawati, S., Musfiroh, I., Muchtaridi, & Fristiohady, A. (2022, December 1). Qust Al Hindi (Saussurea lappa): A Narrative Review Of Its Phytochemistry And Pharmacological Potential Against Covid-19. International Journal of Applied Pharmaceutics. Innovare Academics Sciences Pvt.

Wahab, Abdul et al., 2015. Kuth (Saussurea lappa L.): A review of its traditional uses, phytochemistry and pharmacological potentials. International Journal of Chemical and Biochemical Sciences (ISSN 2226-9614)

Velazquez, E., Tournie, HA., Buschiazzo Mordujovich de, P., Saavedra, G., Schinella, GR. Antioxidant Activity of Paraguayan Plant Extract, Fitoterapia, 2003, 74, 91-97

Zahara K, Tabassum S, Sabir S, Arshad M, Qureshi R, Amjad M et al. A review of therapeutic potential of Saussurea lappa- An endangered plant from Himalaya. Asian Pac J Trop Med 2014; 7(1):S60-S69.

Unduhan

Diterbitkan

2024-10-27

Terbitan

Vol 3 No 2 (2024): (October) Pharmaceutical Reports

Bagian

Articles

Lisensi

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