In Vitro Anti-Inflammatory Activity of Bajakah Tampala Root (Spatholobus littoralis Hassk.)

Sri Karina Maskur, Masdiana Tahir, Rais Razak

Abstract


Bajakah tampala root (Spatholobus littoralis Hassk.) contains phenolic compounds, flavonoids, tannins, terpenoids and saponins. Flavonoid compounds have pharmacological effects, namely anti-inflammatory. The purpose of this study was to determine the anti-inflammatory activity of ethanol extract of bajakah tampala root by inhibiting protein denaturation in vitro using UV-Vis spectrophotometer and diclofenac sodium as positive control. The extract was prepared by maceration method using 96% ethanol solvent. The results showed that the ethanol extract of bajakah tampala root had a percentage of inhibition of 31.27% at the lowest concentration (10 ppm) and 43.28% at the highest concentration (50 ppm) with an IC50 value of 77.92 µg/mL and the ic50 value of diclofenac sodium is 12.26 µg/mL. The percentage of inhibition that is more than 20% is considered to have inhibitory power in the protein denaturation process. So, it can be concluded that the ethanol extract of bajakah root has potential as an anti-inflammatory.

Keywords


Bajakah tampala root; extract; anti-inflammatory; protein denaturation

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References


Angraini, N. and Yanti, F., 2021. Penggunaan spektrofotometer Uv-Vis untuk analisis nutrien fosfat pada sedimen dalam rangka pengembangan modul praktikum oseanografi kimia. Jurnal Penelitian Sains, 23(2), pp.78-83.

Anshari, I., 2012. Isolasi Dan Identifikasi Senyawa Kimia Fraksi Etil Asetat Batang Bajakah Tampala (Spatholobus Littoralis Hassk.) Asal Kalimantan Tengah. Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam Jurusan Farmasi. Universitas Lambung Mangkurat. Banjarbaru.

Bare, Y., Timba, F. N. S., Sari, D. R. T., Mago, O. Y. T., & Nurak, M. M. D. (2022). Kajian in silico Penghambatan Spesifik 3C-like Protease SARS-CoV-2 Senyawa Quinic Acid, Gluconic Acid, dan Ferulic Acid pada Kulit Kopi. Jurnal Ilmiah Medicamento, 8(2), 93-98.

Farida, Y., Rahmat, D., Amanda, A, W. (2018). Uji Aktivitas Antiinflamasi Nanopartikel Ekstrak Etanol Rimpang Temulawak (Curcuma xabthorriza Roxb) Dengan Metode Penghambatan Denaturasi Protein. Jurnal Farmasi. Vol 6.

Fawwaz M, Pratama M, Latu S, Rahayu R, Nurkamilah A. The Potential of Bitter Leaf (Vernonia amygdalina) in Herbal Medicine as Anti-Inflammatory Agent. Industria: Jurnal Teknologi dan Manajemen Agroindustri. 2023; 12(1): 36-44.

Fawwaz M, Muflihunna A, Pratama M, Rahmawati R, Razak R, Baits M. Total phenolic and flavonoid compound of crude and purified extract of green tea leaves (Camellia sinensis) from Makassar-Indonesia. Jurnal Fitofarmaka Indonesia. 2022; 9(3): 19-24.

Hariana, H.A., 2013. 262 Tumbuhan Obat dan Khasiatnya. Penebar Swadaya Grup

Ginwala, R., Bhavsar, R., Chigbu, D.G.I., Jain, P. and Khan, Z.K., 2019. Potential role of flavonoids in treating chronic inflammatory diseases with a special focus on the anti-inflammatory activity of apigenin. Antioxidants, 8(2), p.35

Jannah, N., Saleh, C., & Pratiwi, D. R. (2020). Skiring Fitokimia Ekstrak Etanol dan Fraksi-Fraksi Daun Alamanda (Allamanda Catharica L.). Prosiding Seminar Nasional Kimia Berwawasan Lingkungan 2020, 81–85.

Katzung B G. Basic Clinical Pharmacology. 14th Ed. North America : Mc Graw Education. 2018. P. 2-8, 642-643.

Mulyani, T., Setyahadi, S. and Wibowo, A.E., 2023. Uji Aktivitas Antiinflamasi Kombinasi Ekstrak Daun Torbangun (Plectranthus amboinicus (Lour.) Spreng.) dan Ekstrak Daun Kelor (Moringa oleifera Lam.) dengan Metode Penghambatan Denaturasi Protein. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia).

Nasution, N. A., Nurilmala, M., & Abdullah, A. 2019. Seahorse Hydrolisate (Hippocampus kuda) and Anti-Inflammatory Activity Test with Protein Denaturation Inhibition Method. Jurnal Perikanan Universitas Gadjah Mada, 21(1), 47-51.

Noval, N., Hakim, A.R. and Irawan, A., 2017, December. Antipyretic Effects of (phaleria macrocarpa (scheff) boerl.) Infusa In Mice Galur Wistar As Animal Model. In 2nd Sari Mulia International Conference on Health and Sciences 2017 (SMICHS 2017)–One Health to Address the Problem of Tropical Infectious Diseases in Indonesia (pp. 359-368). Atlantis Press.

Novika, D. S., Ahsanunnisa, R., & Yani, D. F. (2021). Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap Penghambatan Denaturasi Protein. Stannum: Jurnal Sains Dan Terapan Kimia, 3(1), 16-22.

Panche, A.N., Diwan, A.D. and Chandra, S.R., 2016. Flavonoids: an overview. Journal of nutritional science, 5, p.e47.

Poela, S., & Hanafiah, A. (2014). Uji Aktivitas Anti Radang Ekstrak Etanol Biji Pinang (Areca catechu L.) Pada Tikus Putih Jantan. Indonesian Journal of Pharmaceutical Science and Technology, 3(1), 8-14

Rahmawati, R., Widiastuti, H., & Sulistya, E. 2020. 'In Vitro Anti-Inflammatory Assay of Bitter Melon (Momordica charantia L.) Ethanol Extract'. Jurnal Fitofarmaka Indonesia, 7(3): 1–4.

Ramadhani, M. A., Hati, A. K., Lukitasari, N. F., & Jusman, A. H. (2020). Skrining Fitokimia Dan Penetapan Kadar Flavonoid Total Serta Fenolik Total Ekstrak Daun Insulin (Tithonia diversifolia) Dengan Maserasi Menggunakan Pelarut Etanol 96%. Indonesian Journal of Pharmacy and Natural Product, 3(1).

Reynaldi, R. and Yani, D.F., 2021. Potensi Anti-Inflamasi Ekstrak Etanol Daun Cocor Bebek (Kalanchoe pinnata L) Terhadap Denaturasi Protein Secara In Vitro. Spin Jurnal Kimia & Pendidikan Kimia, 3(1), pp.12-21.

Sukandiarsyah, F., Purwaningsih, I., & Ratnawaty, G. J. (2023). Aktivitas Antioksidan Ekstrak Rimpang Temu Ireng (Curcuma aeruginosa Roxb.) Metode DPPH. Jurnal Mandala Pharmacon Indonesia, 9(1), 62-70.

Tukiran, T., Sutoyo, S., Sabila, F. I., & Sari, A. K. (2023). Kadar Total Flavonoid dan Aktivitas Antiinflamasi Kombinasi Ekstrak Etanol Secang (Caesalpinia sappan L.) dan Jahe Merah (Zingiber officinale Roxb.) Terhadap Penghambatan Denaturasi Protein Bovien Serum Albumin. JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya, 7(1), 31-39.




DOI: https://doi.org/10.33096/pharmrep.v4i1.358

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Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy
Universitas Muslim Indonesia, Jl. Urip Sumohardjo KM 5 Kampus II, Makassar 90231, Indonesia
pharmaceutical.reports@umi.ac.id 

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