In Vitro Activity Test of A-Glucosidase Enzyme Inhibition of Moringa oleifera L. Leaves Extract

Irma Pattola, St. Maryam, Rais Razak

Abstract


Diabetes Mellitus (DM) is a group of disease characterized by an increase in insulin. Moringa oleifera leaves contain chemical properties such as tannins, steroids, triterpenes, flavonoids, saponins, anthraquinones and alkaloids. These chemical compounds can affect a variety of biological activities and one of the is antidiabetic. The research aimed to study the potential of Moringa oleifera extract in relation to the a-glucosidase enzyme which is one of the transitions of antidiabetic drugs based on IC50 values. In this study moringa leaves were extracted by maceration method using 96% ethanol solvent. The inhibitory activity testing uses an ELISA (Enzyme-linked Immunosorbent Assay) reader measured at 405 nm wavelength with a comparator of the acarbose because it is an antidiabetic drug of the glucosidase enzyme inhibitor group. The results of the a-glucosidase enzyme showed that the a-glucosidase enzyme activity was inhibited by the ethanol extract of Moringa oleifera L. with C50 value 84,238 µg/mL included in the less active category.

Keywords


Moringa oleifera L.; Enzyme α-glucosidase; IC50; In vitro

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References


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DOI: https://doi.org/10.33096/pharmrep.v1i1.155

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Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy
Universitas Muslim Indonesia, Jl. Urip Sumohardjo KM 5 Kampus II, Makassar 90231, Indonesia
pharmaceutical.reports@umi.ac.id 

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