Chromatogram Profile of Vigna radiata and Phaseolus vulgaris Related to Chemical Hydrolysis

Muammar Fawwaz

Abstract


Mungbean (Vigna radiate) and red bean (Phaseolus vulgaris) is a type of food containing isoflavone in the form of glycoside (genistin, daidzin, glycitin). Isoflavones are one type of phytoestrogen which have similar chemical structure with estradiol. Therefore, it can be used not only to inhibit but also to prevent many symptoms related to estrogen deficiency. Human body cannot adsorb isoflavone in the glycoside form. In order to make it absorbable, isoflavone should be hydrolyzed to defuse its glycoside chain to be aglycon (genistein, daidzein, glycitein). The aim of this study was to determine the influence of hydrolysis on chromatogram profile of mungbean and red bean. Hydrolysis was conducted by chemical method using hydrochloride acid (HCl). The chromatogram profile was determined by High Performance Liquid Chromatography method (HPLC) using C18 reverse phase column, the mobile phase was methanol : water (7:3), the sample injected automatically as much as 10 ml, the flow rate of 1 ml/min with a temperature of 28°C, at a wavelength of 254 nm. This study showed that chemical hydrolysis successes to remove the glycoside chain in both mungbean and red bean extract.

Keywords


Vigna radiate; Phaseolus vulgaris; isoflavone, glycoside; High Performance Liquid Chromatography

Full Text:

PDF

References


Anderson, J.W. Meta-analysis of the effect of soy proteins intake of serum lipid. N Engl J Med. 2002; 333: 276–282.

Blake, D and Winyard, P.G. Immunopharmacology of free radical species, Academic Press, San Diego, USA, 1995.

Department of Health RI. Indonesia Health Profile, Jakarta, Indonesia, 2007.

Donkor, O.N and Shah, N.P. Production of β‐Glucosidase and Hydrolysis of Isoflavone Phytoestrogens by Lactobacillus acidophilus, Bifidobacterium lactis, and Lactobacillus casei in Soymilk. J Food Sci. 2008; 73(1): 15- 20.

Fawwaz, M and Wahyuni. Osteoblast cell proliferation activity of Isoflavone aglycones from fermented soybean (Glycine max (Linn.) Merrill) by Lactobacillus acidophilus. J Chem Pharm Res. 2015; 7 (1): 781-784.

Fawwaz, M and Baits, M. Chemical Hydrolysis of Soybean (Glycine max (L.) Merrill) to Get Genistein Compound. Int J PharmTech Res. 2016; 9(4): 340-343.

Friedman, M and Brandon, D.L. Nutritional and health benefits of soy protein. J Agr Food Chem. 2001; 49: 1069–1086.

Halliwell, B and Gutteridge, J.M.C. In: Free Radicals in Biology and Medicine; Oxford University Press: Oxford, UK, 1998.

Iswandari, R. S.Si Thesis. Isoflavone Content Study on Green Beans, Green Bean Tempe, and Green Bean Puree, Faculty of Agriculture, Bogor, 2008.

Marcelia, K and Martha, I.K. The Effect of Red Peanut Yoghurt on Total Cholesterol Levels in Women Dyslipidemia, J Nutr Coll, 2015; 4: 79 – 88.

Pangastuti, H.A., Affandi, D.R., Ishartani, D. Physical and Chemical Properties Characterization of Red Kidney Bean. J Teknosains Pangan, 2013; 2 (1): 20-29.

Purwoko, T. Isoflavone Aglyon content in Tempe Fermentation Results Rhizopus microspores var. oligosporus: The effect of immersion, BioSMART, 2004; 6(2): 85-87.

Pietta, P.G. Flavonoids and antioxidants. J Nat Prod. 2000; 63: 1035-1042.

Rowland, I., Faughnan, M., Hoey, L., Wähälä, K., Williamson, G., Cassidy, A. Bioavailability of phytoestrogens, BR J Nutr. 2003; 89 (S1): S45-S58.

Schmidl, M.K and Labuza, T.P. Essential of Functional Foods. Aspen Publisher, Inc: Gaithersburg, Maryland, 2000.

Uesugi, T., Fukui, Y., Yamori Y. Beneficial Effects of Soybean Isoflavone Supplementation on Bone Metabolism and Serum Lipids in Postmenopausal Japanese Women: A Four-Week Study. J Am Coll Nutr. 2002; 21(2): 97–102.

Zhang, E.J., Ming, K., Luo, K.Q. Extraction and Purification of Isoflavones from Soybeans and Characterization of Their Estrogenic Activities, University of Science and Technology, Hongkong, 2007.

Zubik, L and Meydani, M. Bioavailability of soybean isoflavone from aglycone and glucoside form in American women. Am J Clin Nutr. 2003; 77: 1459-1465.




DOI: https://doi.org/10.33096/pharmrep.v1i1.153

Refbacks

  • There are currently no refbacks.


 

Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy
Universitas Muslim Indonesia, Jl. Urip Sumohardjo KM 5 Kampus II, Makassar 90231, Indonesia
pharmaceutical.reports@umi.ac.id 

 Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Indexed By: