Determination of Tannin Content of Gringsingan Stem Extract (Mesosphaerum suaveolens (L) Kuntze) by the UV-Vis Spectrophotometry

Selpida Handayani, Aminah Aminah, Indah Nurliadi

Abstract


The Gringsingan plant (Mesosphaerum suaveolens (L.) Kuntze) is widely used in traditional medicine and is known for its diverse pharmacological properties. Traditionally, it has been utilized to treat various ailments such as tumors, malaria, headaches, cancer, fever, stomach pain, yellow fever, rheumatism, constipation, urethritis, diarrhea, and kidney disorders, as well as to act as an analgesic, antiseptic, antispasmodic, and depurative agent. The present study aimed to determine the total tannin content in the stem extract of Gringsingan obtained through Microwave-Assisted Extraction (MAE) and to evaluate its potential as a bioactive compound source. Microwave-assisted extraction was selected because of its advantages, including shorter extraction time, reduced solvent consumption, and efficient recovery of active compounds compared to conventional methods. This experimental research was conducted in the laboratory and consisted of several stages: sample preparation, qualitative testing for tannin presence, and quantitative determination of total tannin content. Qualitative identification using 1% FeCl₃ reagent produced a dark blue to greenish-black coloration, confirming the presence of tannins in the Gringsingan stem extract. The result was further supported by thin-layer chromatography (TLC) testing with the same reagent, which yielded consistent positive results. Quantitative analysis of the total tannin content was carried out using UV–Visible spectrophotometry, with absorbance measured at the maximum wavelength for gallic acid. The analysis revealed that the tannin concentration in the Gringsingan stem extract was 7.487 mg GAE/g extract, indicating that this plant possesses measurable levels of tannins that contribute to its traditional medicinal value.

Keywords


Gringsingan; extraction; phytochemical; spectrophotometry; tannin

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References


Amelia, R.F. Penentuan Jenis Tanin dan Penetapan Kadar Tanin dari Buah Bungur Muda (Lagerstroemia speciosa Pers.) secara Spektrofotometri dan Permanganometri. Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2015; 4(2), 1.

Ardin, N.C., Pratama, M., Widiastuti, H. and Fawwaz, M. Total Flavonoid Content of the Bark Extract of Salacca edulis Reinw. Pharmaceutical Reports, 2022; 1(2).

Danong, M. T., Hero, L. R., Studi, P., & Fst, B. (2021). Analısıs Senyawa Metabolıt Sekunder Batang Grıngsıngan ( Hyptis suaveolens L . Poit .) Asal Kota Kupang. 18(3), 18–27.

Egra, S., Mardhiana, ., Rofin, M., Adiwena, M., Jannah, N., Kuspradini, H., & Mitsunaga, T. Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia Solanacearum Penyebab Penyakit Layu. Agrovigor: Jurnal Agroekoteknologi, 2019; 12(1), 26. https://doi.org/10.21107/agrovigor.v12i1.5143

Enggiwanto, S., Istiqomah, F., Daniati, K., Roanisca, O., & Mahardika, R. G. Ekstraksi Daun Pelawan (Tristaniopsis merguensis Griff.) Dengan Metode Microwave Assisted Extraction Dan Uji Fitokimianya. Prosiding Seminar Nasional Penelitian & Pengabdian Pada Masyarakat, 1–3. 2018.

Elfirta, R., Saskiawan, I., Kasirah, Rahadian Amalia, R. L., Zahrah Nadhirah Ikhwani, A., & Widhyastuti, N. Kadar Karbohidrat dan Penapisan Senyawa Aktif dari Pleurotus pulmonarius yang Diekstraksi Menggunakan Metode Microwave Assisted Extraction (MAE) dengan Beberapa Perlakuan Suhu. Jurnal Biologi Indonesia, 2023; 19(1), 65–76. https://doi.org/10.47349/jbi/19012023/65.

Fawwaz, M., Muflihunna, A., Pratama, M., Rahmawati, R., Razak, R. and Baits, M. Total phenolic and flavonoid compound of crude and purified extract of green tea leaves (Camellia sinensis) from Makassar-Indonesia. Jurnal Fitofarmaka Indonesia, 2022; 9(3), pp.19-24.

Fatonah, R., Mulyaningsih, S., & Ardiana, C. Penentuan Kadar Total Tanin dari Ekstrak Daun Binahong (Anredera cordifolia). Jurnal Life Science: Jurnal Pendidikan Dan Ilmu Pengetahuan Alam, 2021; 3(2), 38–46. https://doi.org/10.31980/jls.v3i2.1670

Forestryana, D., & Arnida, A. Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Daun Jeruju (Hydrolea Spinosa L.). Jurnal Ilmiah Farmako Bahari, 2020; 11(2), 113. https://doi.org/10.52434/jfb.v11i2.859

Huda, R. N., Pratiwi, E., & Kunarto, B. Ekstraksi oleoresin kencur (Kaempferia Galanga) pada berbagai lama microwave assisted extraction. Teknologi Hasil Pertanian, 2018; 5–24.

Iriany, A.H., & Namira, C. A. Jurnal Teknık Kımıa-Usu Ekstraksi Tanin dari Buah Balakka (Phyllanthus emblica L.) dengan Bantuan Microwave: Pengaruh Daya Microwave, Perbandingan Massa Kering Terhadap Jumlah Pelarut Etil Asetat Extraction of Tannin from Indian Gooseberry (Phyllanthus em. Jurnal Teknik Kimia USU, 2021; 10(1), 8–12. https://talenta.usu.ac.id/jtk

Kusumo, G. G., Fernanda, F.M.A. and Asroriyah, H. Identifikasi Senyawa Tanin Pada Daun Kemuning (Murraya panicullata L. Jack) Dengan Berbagai Jenis Pelarut Pengekstraksi. Journal of Pharmacy and Science, 2017; 2(1), 29–32. https://doi.org/10.53342/pharmasci.v2i1.63

Lestari, T., & Sidik, Y. Isolası dan Identıfıkası Senyawa Tanın Darı Ekstrak Aır Kulıt Batang Kelapa Gadıng (Cocos nucifera var. eburnea). 2013; 9.

Listiana, L., Wahlanto, P., Ramadhani, S. S., & Ismail, R. Penetapan Kadar Tanin Dalam Daun Mangkokan (Nothopanax scutellarium Merr) Perasan Dan Rebusan Dengan Spektrofotometer UV-Vis. Pharmacy Genius, 2022; 1(1), 62–73. https://doi.org/10.56359/pharmgen.v1i01.152

Maulida, S., Hakim, A. R., & Mohtar, M. S. Analisis Kadar Tanin Ekstrak Etanol Kulit Batang Kemiri (Aleurites moluccana (L.) Willd) Dengan Metode Titrimetri. Journal of Pharmaceutical Care and Science, 2020; 1(1), 85–93.

Pratini, C. E. Ekstraksi Tanin dari Kulit Kayu Pinus dengan Bantuan Microwave:Pengaruh Daya Microwave, Jenis Pelarut dan Waktu ekstraksi. Jurnal Integrasi Proses, 2017; 6(4), 155. https://doi.org/10.36055/jip.v6i4.2429

Pratiwi, S. A., Februyani, N., Basith, A., Program, ), Fakultas, S. F., Kesehatan, I., Nahdlatul, U., Sunan, U., Bojonegoro, G., Yani, A., 10, N., Bojonegoro, K., Timur, J., & Boojonegoro, K. Skrining dan Uji Penggolongan Fitokimia dengan Metode KLT pada Ekstrak Etanol Kemangi (Ocium basilicum L) dan Sereh Dapur (Cymbopogon ciratus). Pharmacy Medical Journal, 2023; 6(2), 2023.

Primayani, A.S., & Chatri, M. Efektivitas Ekstrak Hyptis suaveolens (L.) Poit. Dalam Menghambat Pertumbuhan Jamur Scelerotium rolfsii secara In-vitro. Bio Sains, 2018; 1(1), 59–66. http://ejournal.unp.ac.id/students/index.php/bio

Shenoy, C., Patil, M. B., & Kumar, R. Wound healing activity of Hyptis suaveolens (L.) Poit (Lamiaceae). International Journal of PharmTech Research, 2009; 1(3), 737–744.




DOI: https://doi.org/10.33096/pharmrep.v4i2.378

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Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy
Universitas Muslim Indonesia, Jl. Urip Sumohardjo KM 5 Kampus II, Makassar 90231, Indonesia
pharmaceutical.reports@umi.ac.id 

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