Determination of Total Flavonoid Content of Red Fruit Juice (Pandanus conoideus Lam) by a UV–Vis Spectrophotometer

Nur Rachmi Sulaeman, Mamat Pratama, Andi Trihadi Kusuma

Abstract


Red fruit (Pandanus conoideus Lam) belongs to the pandanacea family. This plant contains compounds such as tannins, flavonoids, steroids, triterpenoids and alkaloids and has properties in curing various diseases such as diabetes, coronary heart disease, hypertension, cancer and HIV/AIDS. This study aims to determine the total flavonoid content of red fruit juice (Pandanus conoideus Lam) using a UV - Vis spectrophotometer. The stages carried out in this research began with sample preparation, qualitative testing of flavonoid compounds and determining the total flavonoid content of red fruit (Pandanus conoideus Lam) samples. The results obtained in this study based on a qualitative test using NaOH reagent stated that red fruit juice (Pandanus conoideus Lam) was positive for containing flavonoids and in a quantitative test using a UV - Vis spectrophotometer the percent concentration obtained in the red fruit juice sample was 8.7258%.


Keywords


Red fruit (Pandanus conoideus Lam.); flavonoids; UV-Vis Spectrophotometer

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References


Bachtiar, A.R. et al. Penetapan kadar flavonoıd total buah dengen (Dillenia serrata) menggunakan metode spektrofotometrı uv-vıs, 2023; 1(2), pp. 86–101.

Fahrudin, F. Percobaan Terhadap Tikus Putih Jantan Galur Spraque Dawley, 2014 ; 7, pp. 89–93.

Fawwaz, M., Pratama, M., Musafira, M., Wahab, I., Iriani, R., Aminah, A., Kusuma, A. T., & Arsul, M. I. (2023). Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content. Indonesian Journal of Pharmaceutical Science and Technology, 10(2), 104.

Jubaidah, S.J. Analısıs Flavonoıd Total Akar Tabar Kedayan (Aristolochia foveolata Merr), Al-Kimia, 2018; 6(2). Available at: https://doi.org/10.24252/al-kimia.v6i2.4996.

Lindawati, N.Y. and Ni’ma, A. Analysis of Total Flavanoid Levels of Fennel Leaves (Foeniculum Vulgare) Ethanol Extract By Spectrophotometry Visibel, Jurnal Farmasi Sains dan Praktis, 2022; 8(1), pp. 1–12. Available at: https://doi.org/10.31603/pharmacy.v8i1.4972.

Maran, P., Siburian, R.H. and Hendri. Morfologi dan karakteristik tempat tumbuh tanaman buah merah (Pandanus conoideus Lamk) di Kampung Eroma Distrik Kurima Kabupaten Yahukimo, Cassowary, 2022; 5(2), pp. 112–119. Available at: https://doi.org/10.30862/casssowary.cs.v5.i2.149.

Maulana K, A. et al. Analisa Aktivitas Antioksidan Ekstrak Biji Nangka (Artocarpus heterophyllus Lam) dengan Metode Frap (Ferric Reducing Antioxidant Power, Bionature, 2019; 20(1), pp. 27–33. Available at: https://doi.org/10.35580/bionature.v20i1.9757.

Mellyana Saa, S. and Prabawati, R. Bıolearnıng Journal Pengaruh Penggunaan Mınyak Buah Merah ( Pandanus Conoideus ) Sebagaı Antıseptık Terhadap Proses Penyembuhan Luka Sayatan Kırı Pada Mencıt ( Mus Musculus ), 2022; 9(2), pp. 2406–8241.

Pratama, M., Baits, M., Ananda. Determination of Total Flavonoid Content of Ethanol Extract of Jongi (Dillenia serrata) Leaf Using Uv - Vis Spectrophotometry Methodr, 2023; 1(3), pp. 224–232.

Sadolona, E. and Agustin, R. Pengaruh Penambahan Minyak Buah Merah Terhadap Kualitas Organoleptik Nugget Ayam’, Jurnal AgroSainTa: Widyaiswara Mandiri Membangun Bangsa, 2021; 5(2), pp. 77–84. Available at: https://doi.org/10.51589/ags.v5i2.73.

Sukmawati, Sudewi, S. and Pontoh, J. Optimasi dan Validasi Metode Analisis Dalam Penentuan Kandungan Total Flavonoid Pada Ekstrak Daun Gedi Hijau (Abelmoscus manihot L.) yang Diukur Menggunakan Spektrofotomter UV-Vis, PHARMACON Jurnal Ilmiah Farmasi-UNSRAT, 2018; 7(3), pp. 32–41.

Wahdaningsih et al., Penetapan Kadar Fenolık Total dan Flavonoıd Total Ekstrak Metanol dan Fraksı Etıl Asetat Kulıt Buah, 2017; 6(3), pp. 295–301.

Widyasari, R. and Handayani, S. Penetapan Kadar Flavonoid Total Ekstrak Metanol Kulit Jeruk Sambal Secara Spektrofotometri Uv-Visibel, Medical Sains : Jurnal Ilmiah Kefarmasian, 2020; 4(2), pp. 111–118. Available at: https://doi.org/10.37874/ms.v4i2.129.

Yanlinastuti and Fatimah, S. Pengaruh Konsentrası Pelarut Untuk Menentukan Kadar Zırkonıum Dalam Paduan U-Zr Dengan Menggunakan Metode Spektrofotometrı Uv-Vıs, Pusat Teknologi Bahan Nuklir, 2016; 9(17), pp. 22–33




DOI: https://doi.org/10.33096/pharmrep.v4i1.360

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Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy
Universitas Muslim Indonesia, Jl. Urip Sumohardjo KM 5 Kampus II, Makassar 90231, Indonesia
pharmaceutical.reports@umi.ac.id 

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