Antibacterial Activity of Actinomycetes Isolates from The Rhizosphere of The Turate Kasumba Plant (Carthamus Tinctorius L.) Against Bacteria Causing Skin Infections

Safira Brigeeta Kinanti, Rachmat Kosman, Rusli Rusli

Abstract


Actinomycetes is one group of microorganisms capable of producing secondary metabolites that function as antibacterials. Actinomycetes isolates used were isolated from the rhizosphere of the kasumba turate plant (Carthamus tinctorius L.). The purpose of this study was to determine the antibacterial activity of Actinomycetes isolates from the rhizosphere of kasumba turate (Carthamus tinctorius L.) which is the largest and most against skin infection bacteria. The results of the study obtained 2 isolates that are most active. IARK-6 obtained spot with Rf value = 0.70 for Staphylococcus aureus bacteria, Rf1 = 0.70 and Rf2 = 0.38 for Staphylococcus epidermidis bacteria, Rf = 0.70 for Pseudomonas aeruginosa bacteria. While the results of KLT-Bioautography testing for isolate IARK-7 obtained spots with a value of Rf = 0.38 for Staphylococcus aureus bacteria, Rf = 0.70 for Staphylococcus epidermidis bacteria, Rf = 0.38 for Pseudomonas aeruginosa bacteria.

Keywords


Antibacterial; Actinomycetes; Kasumba Turate Rhizosphere; KLT-Bioautography; Carthamus tinctorius L

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References


Agustina D, Mustafidah H, Purbowati MR, Expert system for diagnosing skin diseases due to fungal infections, JUITA, Journal of Informatics, 2017.

Ali A, Screening and characterization of persian antifungal compounds from actinomycetes origin of decomposed sago solid waste, Berk Biological Research, 2009.

Ambarwati A, CJ Soegihardjo, Sembiring L, Isolation and identification of streptomycetes from the corn rizosphere (Zea mays L.) that have the potential to produce antibiotics, Biota Journal, 2010.

Davis WW and TR Stout, Disc plate methods of microbiological antibiotic assay, Microbiology, 1971.

Djata IMR, Setyobudy A, Hinga IAT, Overview of environmental sanitation and personal hygiene with the incidence of skin disease in kupang city children's prison, SEHATMAS: Scientific Journal of Public Health, 2022.

Djide MN, and Sartini, Pharmaceutical Microbiology Analysis. Laboratory of Pharmaceutical Microbiology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, 2008.

Dwijoseputro D, Basics of Food Microbiology, PT Raja Grafindo Persada, Jakarta, 1998.

Fitriana and Rusli, Determination of the optimum time of production of secondary metabolites of bacterial isolates of actinomycetes from the soil rhizosphere of jatropha plant roots (Jatropha curcas L.) against pathogenic bacteria, As-Syifaa Scientific Journal, 2018.

Oka Suyasa IB, Isolation and morphological characterization of bacterial colonies in the digestive tract of grouper (Cephalopolies miniata) fro the waters of klungkung regency bali, Meditory: The Journal of Medical Laboratory, 2019.

Putri VA, Posangi J, Nangoy E, Bara RA, Test of the inhibitory power of endophytic fungi of galangal rhizomes (Alpinia galanga L.) against the growth of Escherichia coli and Staphylococcus aureus bacteria, Ebiomedic, 2016.

Riyanti, Aziz S, Sabdono A, Radjasa K, Detection of NPRS actinomycetes gene of seaweed and soft coral symbionts, proceedings of the national seminar, Sustainable Development of Rural Resources and Local Wisdom II, 2012.

Rusli, Kosman R, Melinda P, Tracing endophytic function in kopasanda leaves (Chromolaena odorata L.) which has the potential to produce antibacterial against batteries that cause skin infections, As-Syifaa Journal of Pharmacy, 2020.

Rohman A, Chromatography for Drug Analysis, Graha Science, Yogyakarta, 2009.

Sulistyani N and Akbar AN, Activity of Actinomycetes Isolate from Seaweed (Eucheuma cottoni) as Antibiotic Producer against Staphylococcus aureus and Escherichia coli, Indonesian Journal of Pharmaceutical Sciences, 2014.

Todar K, Online Textbook of Microbiology, Madison, Wisconsin, 2009.




DOI: https://doi.org/10.33096/pharmrep.v2i1.226

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